Investigation of In-Vitro Antidiabetic Study, Antioxidant Activity and Anthelminthic Property of Various Extracts of Bitter Cumin Seeds
Antidiabetic Study, Antioxidant Activity, and Anthelminthic Property of Bitter Cumin Seeds
Bitter cumin (Centratherum anthelminticum (L.)) is a significant restorative plant. We examined the phenolic compounds, and antioxidants, hostile to hyperglycemic and anthelmintic properties of bitter cumin extracts in different in vitro models.
Bitter cumin seeds were extracted with various solvents in their rising request of extremity, which incorporates chloroform, ethyl acetate, methanol, ethanol, and distilled water. The -amylase test and glucose take-up measure by yeast were used for the assessment of the antidiabetic property. Antioxidant agent actions of bitter cumin extricate were described in different in vitro model frameworks, for example, DPPH Scavenging Effect, Ferric-Reducing Antioxidant Power, and Metal-Chelating Capacity. Phenolic content was likewise assessed and, in conclusion, the in vitro anthelmintic review was completed by utilizing Phertima posthuma.
The antidiabetic property of the different concentrates of bitter cumin uncovered the best action from the fluid concentrate by α-amylase action with a hindrance worth of 39.2830±0.80725%. While the investigation of glucose take-up by yeast cells was the most noteworthy, the restraint worth of the watery concentrate was the most noteworthy at 49.8100±0.62476%. The different concentrates of bitter cumin in an in-vitro study showed important properties of DPPH Ferric-Reducing Antioxidant Power and Metal-Chelating Capacity. The outcomes showed an immediate relationship between phenolic correlation substances and cell reinforcement action. The anthelmintic review uncovered that the fluid concentrate from the wide range of various concentrates was having the most incredible in-vitro action, which uncovered that the time required was 3.52±0.158 min for the loss of motility and 2.74±0.247 min for the death of the earthworms.
The exploratory proof obtained in the laboratory model could give a reason for the conventional utilization of this seed as an antidiabetic, cell-protective, and anthelmintic. Our discoveries affirm that the customary restorative cases for this seed in the not-so-distant future certainly have the option to supplant the manufactured medications to which there is an increased occurrence of medication, increased incidence of drug interactions, and drug resistance.
Keywords:α-amylase assay, In-vitro anti-diabetic activity, Bitter cumin, Antioxidant Activity, Anthelminthic Property, Glucose Uptake Assay
How to Cite
Malviya N, Jain S, Malviya SA. Antidiabetic potential of medicinal plants. Acta pol pharm. 2010 Mar 1;67(2):113-8.
Shukia R, Sharma SB, Puri D, Prabhu KM, Murthy PS. Medicinal plants for treatment of diabetes mellitus. Indian Journal of Clinical Biochemistry. 2000 Aug;15:169-77.
Kaul K, Tarr JM, Ahmad SI, Kohner EM, Chibber R. Introduction to diabetes mellitus. Diabetes: an old disease, a new insight. 2013:1-1.
Gerich JE. Oral hypoglycemic agents. New England Journal of Medicine. 1989 Nov 2;321(18):1231-45.
Sabu MC, Kuttan R. Antidiabetic activity of medicinal plants and its relationship with their antioxidant property. Journal of ethnopharmacology. 2002 Jul 1;81(2):155-60.
Decker EA, Akoh CC, Min DB. Food lipids: chemistry, nutrition and biotechnology. Antioxidant Mechanisms. 1998:397-421.
Smith MA, Perry G, Richey PL, Sayrec LM, Anderson VE, Beal MF, Kowall N. Oxidative damage in Alzheimer's. Nature. 1996 Jul 11;382(6587):120-1.
Neumann CA, Krause DS, Carman CV, Das S, Dubey DP, Abraham JL, Bronson RT, Fujiwara Y, Orkin SH, Van Etten RA. Essential role for the peroxiredoxin Prdx1 in erythrocyte antioxidant defence and tumour suppression. Nature. 2003 Jul 31;424(6948):561-5.
Zenebe S, Feyera T, Assefa S. In vitro anthelmintic activity of crude extracts of aerial parts of Cissus quadrangularis L. and leaves of Schinus molle L. against Haemonchus contortus. BioMed research international. 2017 Dec 19;2017.
Prichard RK. Anthelmintic resistance in nematodes: extent, recent understanding and future directions for control and research. International journal for parasitology. 1990 Jul 1;20(4):515-23.
Wakayo BU, Dewo TF. Anthelmintic resistance of gastrointestinal parasites in small ruminants: a review of the case of Ethiopia. J Veterinar Sci Technol S. 2015;10(4).
Amir F, Chin KY. The chemical constituents and pharmacology of Centratherum anthelminticum. Int J PharmTech Res. 2011;3:1772-9.
De Castro ML, Priego-Capote F. Soxhlet extraction: Past and present panacea. Journal of chromatography A. 2010 Apr 16;1217(16):2383-9.
López-Bascón MA, De Castro ML. Soxhlet extraction. InLiquid-phase extraction 2020 Jan 1 (pp. 327-354). Elsevier.
Debella A. Manual for phytochemical screening of medicinal plants. Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia. 2002;76(5):35-47.
Mueller MS, Mechler E. Medicinal plants in tropical countries. Traditional use-experience-facts. Georg Thieme Verlag; 2005.
Ranjit, P. M., Santhipriya, T., Nagasri, S., Chowdary, Y., Pasumarthy, N., & Gopal, V. Preliminary phytochemical screening and antibacterial activities of ethanolic extract of Calotropis procera flowers against human pathogenic strains. Asian J Pharm Clin Res, 2012; 5(3), 127-31.
Bhandari MR, Jong-Anurakkun N, Hong G, Kawabata J. α-Glucosidase and α-amylase inhibitory activities of Nepalese medicinal herb Pakhanbhed (Bergenia ciliata, Haw.). Food Chemistry. 2008 Jan 1;106(1):247-52.
Jung M, Park M, Lee HC, Kang YH, Kang ES, Kim SK. Antidiabetic agents from medicinal plants. Current medicinal chemistry. 2006 Apr 1;13(10):1203-18.
Khadayat K, Marasini BP, Gautam H, Ghaju S, Parajuli N. Evaluation of the alpha-amylase inhibitory activity of Nepalese medicinal plants used in the treatment of diabetes mellitus. Clinical Phytoscience. 2020 Dec;6(1):1-8.
Adisakwattana S, Ruengsamran T, Kampa P, Sompong W. In vitro inhibitory effects of plant-based foods and their combinations on intestinal α-glucosidase and pancreatic α-amylase. BMC complementary and alternative medicine. 2012 Dec;12(1):1-8.
Mayur B, Sandesh S, Shruti S, Sung-Yum S. Antioxidant and α-glucosidase inhibitory properties of Carpesium abrotanoides L. Journal of Medicinal Plants Research. 2010 Aug 4;4(15):1547-53.
Mirsky N, Weiss A, Dori Z. The effect of glucose tolerance factor on glucose uptake by yeast cells. Journal of inorganic biochemistry. 1981 Jan 1;15(3):275-9.
Pitchaipillai R, Ponniah T. In vitro antidiabetic activity of ethanolic leaf extract of bruguiera Cylindrica L.–glucose uptake by yeast cells method. International Biological and Biomedical Journal. 2016 Dec 10;2(4):171-5.
Faiyaz A, Sudha S, Asna U. Effect of various ayurvedic formulations and medicinal plants on carbohydrate hydrolyzing enzymes and glucose uptake by yeast cells-an in vitro study. Journal of Pharmacy Research. 2009;2(3):563-8.
Madagi SB, Hoskeri JH, Vedamurthy AB. Phytochemical profiling, in-vitro antioxidant and anti-inflammatory activities of Hopea ponga, Kandelia candel, Vitex leucoxylon and Rhizophora apiculata. Journal of Pharmacognosy and Phytochemistry. 2018;7(6):1425-40.
Sunil C, Agastian P, Kumarappan C, Ignacimuthu S. In vitro antioxidant, antidiabetic and antilipidemic activities of Symplocos cochinchinensis (Lour.) S. Moore bark. Food and chemical toxicology. 2012 May 1;50(5):1547-53.
Benzie IF, Szeto YT. Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. Journal of agricultural and food chemistry. 1999 Feb 15;47(2):633-6.
Pulido R, Bravo L, Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of agricultural and food chemistry. 2000 Aug 21;48(8):3396-402.
Gulcin İ, Alwasel SH. Metal ions, metal chelators and metal chelating assay as antioxidant method. Processes. 2022 Jan 10;10(1):132.
Canabady-Rochelle LL, Harscoat-Schiavo C, Kessler V, Aymes A, Fournier F, Girardet JM. Determination of reducing power and metal chelating ability of antioxidant peptides: Revisited methods. Food Chemistry. 2015 Sep 15;183:129-35.
Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature protocols. 2007 Apr;2(4):875-7.
Shi JY, Zou XB, Zhao JW, Mel H, Wang KL, Wang X, Chen H. Determination of total flavonoids content in fresh Ginkgo biloba leaf with different colors using near infrared spectroscopy. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2012 Aug 1;94:271-6.
Soetan KO, Lasisi OT, Agboluaje AK. Comparative assessment of in-vitro anthelmintic effects of the aqueous extracts of the seeds and leaves of the African locust bean (Parkia biglobosa) on bovine nematode eggs. Journal of Cell and Animal Biology. 2011 Jun;5(6):109-12.
Alawa CB, Adamu AM, Gefu JO, Ajanusi OJ, Abdu PA, Chiezey NP, Alawa JN, Bowman DD. In vitro screening of two Nigerian medicinal plants (Vernonia amygdalina and Annona senegalensis) for anthelmintic activity. Veterinary parasitology. 2003 Apr 2;113(1):73-81.
Silva VC, Carvalho MG, Borba HR, Silva SL. Anthelmintic activity of flavonoids isolated from roots of Andira anthelmia (Leguminosae). Revista Brasileira de Farmacognosia. 2008;18:573-6.