Investigation of In-Vitro Antidiabetic Study, Antioxidant Activity and Anthelminthic Property of Various Extracts of Bitter Cumin Seeds

Antidiabetic Study, Antioxidant Activity, and Anthelminthic Property of Bitter Cumin Seeds



  • Vedanshu Malviya Department of Pharmaceutics, P.R. Pote Patil College of Pharmacy, Amravati-444604



Bitter cumin (Centratherum anthelminticum (L.)) is a significant restorative plant. We examined the phenolic compounds, and antioxidants, hostile to hyperglycemic and anthelmintic properties of bitter cumin extracts in different in vitro models.


Bitter cumin seeds were extracted with various solvents in their rising request of extremity, which incorporates chloroform, ethyl acetate, methanol, ethanol, and distilled water. The -amylase test and glucose take-up measure by yeast were used for the assessment of the antidiabetic property. Antioxidant agent actions of bitter cumin extricate were described in different in vitro model frameworks, for example, DPPH Scavenging Effect, Ferric-Reducing Antioxidant Power, and Metal-Chelating Capacity. Phenolic content was likewise assessed and, in conclusion, the in vitro anthelmintic review was completed by utilizing Phertima posthuma.


The antidiabetic property of the different concentrates of bitter cumin uncovered the best action from the fluid concentrate by α-amylase action with a hindrance worth of 39.2830±0.80725%. While the investigation of glucose take-up by yeast cells was the most noteworthy, the restraint worth of the watery concentrate was the most noteworthy at 49.8100±0.62476%. The different concentrates of bitter cumin in an in-vitro study showed important properties of DPPH Ferric-Reducing Antioxidant Power and Metal-Chelating Capacity. The outcomes showed an immediate relationship between phenolic correlation substances and cell reinforcement action. The anthelmintic review uncovered that the fluid concentrate from the wide range of various concentrates was having the most incredible in-vitro action, which uncovered that the time required was 3.52±0.158 min for the loss of motility and 2.74±0.247 min for the death of the earthworms.


The exploratory proof obtained in the laboratory model could give a reason for the conventional utilization of this seed as an antidiabetic, cell-protective, and anthelmintic. Our discoveries affirm that the customary restorative cases for this seed in the not-so-distant future certainly have the option to supplant the manufactured medications to which there is an increased occurrence of medication, increased incidence of drug interactions, and drug resistance.


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α-amylase assay, In-vitro anti-diabetic activity, Bitter cumin, Antioxidant Activity, Anthelminthic Property, Glucose Uptake Assay

Author Biography

Vedanshu Malviya, Department of Pharmaceutics, P.R. Pote Patil College of Pharmacy, Amravati-444604

Department of Pharmaceutics, P.R. Pote Patil College of Pharmacy, Amravati-444604



How to Cite

Malviya V. Investigation of In-Vitro Antidiabetic Study, Antioxidant Activity and Anthelminthic Property of Various Extracts of Bitter Cumin Seeds: Antidiabetic Study, Antioxidant Activity, and Anthelminthic Property of Bitter Cumin Seeds. Scopus Indexed [Internet]. 2023 Jul. 31 [cited 2023 Dec. 11];16(4):6855-74. Available from:



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