Role of Temperature on the Size of Niosomes Formed by the Ether Injection Method



  • Shweta Agarwal
  • Pallavi Tempta
  • Poonam Kaushik
  • Kusum Kaushik


Niosomes are lamellar vesicles formed by self-association of non-ionic surfactants and cholesterol having good compatibility with the biological system and low toxicity. Their ability to encapsulate both hydrophilic and hydrophobic drugs, their potential use in protection of drugs, controlled release and targeted drug delivery makes them a promising drug delivery system. Various factors like nature of drug, amount and type of surfactant used, method of preparation, amount and charge of cholesterol used, type and volume of hydrating medium, speed of agitation, time and temperature of hydration medium affect the type and characteristics of niosomes formed. Therefore, a study was conducted to determine the effect of hydration temperature on the size of niosomes formed by ether injection method. Aceclofenac was used as the model drug as it is lipophilic and shows good encapsulation in niosomes. Span 20 was used as the non-ionic surfactant. The study was conducted at three temperatures of hydration and all the other variables were kept constant. The niosomes formed at the different hydration temperatures were evaluated for shape and size by optical microscopy and mean vesicle size determined at each temperature. The data obtained for size was statistically analysed by the software Graphpad prism version 7 using ordinary one-way ANOVA. The p value obtained (at 95% confidence interval: p< 0.05) was used to determine significant difference between the mean vesicle sizes at the three temperatures. The study showed that hydration temperature played a major role in the size of niosomes formed by ether injection method.


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Niosomes, Aceclofenac, Span20, Hydration, Encapsulation, Vesicle size




How to Cite

Agarwal S, Tempta P, Kaushik P, Kaushik K. Role of Temperature on the Size of Niosomes Formed by the Ether Injection Method . Scopus Indexed [Internet]. 2019 May 31 [cited 2024 Apr. 20];12(3):4559-64. Available from:



Research Articles


Arslan SA and Tirnaksiz F (2010). A Nonsteroidal Anti-inflammatory Drug: Aceclofenac. J. Pharm. Sci 35: 105-118.
Arunothayanun P, Bernard MS, Craig DQ, Uchegbu IF, and Florence AT (2000). The effect of processing variables on the physical characteristics of nonionic surfactant vesicles (niosomes) formed from hexadecyl diglycerol ether. Int J Pharm 201: 7-14.
Asthana GS, Sharma PK, and Asthana A (2016). In Vitro and In Vivo Evaluation of Niosomal Formulation for Controlled Delivery of Clarithromycin. Scientifica 20: 1-10.
Biswal S, Murthy PN, Sahu J, Sahoo P, and Amir F (2008). Vesicles of Non-ionic Surfactants (Niosomes) and Drug Delivery Potential. Int J Pharm Sci Nanotech 1: 1-8.
Essa EA (2010). Effect of formulation and processing variables on the particle size of sorbitan monopalmitate niosomes. Asian Journal of Pharmaceutics 4(4): 227-233.
Firthouse PUM, Halith SM, Wahab SU, Sirajudeen M, and Mohideen SK (2011). Formulation and evaluation of miconazole niosomes. International Journal of PharmTech Research. 3(2): 1019-1022.
Homaei M (2016). Preparation and characterization of giant niosomes. Masters Thesis, Chalmers University of Technology. Gothenburg, Sweden.
Kazi KM, Mandal AS, Biswas N, Guha A, Chatterjee S, Behera M, and Kuotsu K (2010). Niosome: A future of targeted drug delivery systems. Journal of Advanced Pharmaceutical Technology & Research. 4(1): 374-380.
Marwa A, Omaima S, Hanaa ELG, and Mohammed AS (2013). Preparation and in-vitro evaluation of diclofenac sodium niosomal formulations. International Journal of Pharmaceutical Sciences and Research 4(5): 1757-1765.
Mishra N, Srivastava V, Kaushik A, chauhan V, and Srivastava G (2014). Formulation and in-vitro evaluation of Niosomes of aceclofenac. Journal of Scientific and Innovative Research 3(3): 337-341.
Ravalika V, and Sailaja AK (2017). Formulation and evaluation of etoricoxib niosomes by thin film hydration technique and ether injection method. Nano Biomed. Eng 9(3): 242-248.
Samifara S, Akki R, Ramya MG, and Naik VV (2015). Development and characterization of carbamazepine loaded nonionic surfactant vesicles. Acta Chim. Pharm. Indica 5(1): 29-40.
Seleci Ag D, Seleci M, Walter JG, Stahl F, and Scheper T (2016). Niosomes as Nanoparticular Drug Carriers: Fundamentals and Recent Applications. Journal of Nanomaterials 2016: 1-13.
Shreedevi HM, Nesalin JAJ, and Mani TT (2016). Development and evaluation of stavudine niosome by ether injection method. International Journal of Pharma Sciences and Research 7: 38-46.
Uchegbu IF, and Vyas SP (1998). Non-ionic surfactant-based vesicles (niosomes) in drug delivery. International Journal of Pharma-ceutics 172: 33-70.