Simultaneous Spectrophotometric Estimation of Azithromycin and Ofloxacin using π-Acceptors as Analytical Reagents
DOI:
https://doi.org/10.37285/ijpsn.2020.13.5.5Abstract
In this study, two sensitive and precise spectrophotometric methods have been developed for the simultaneous determination of azithromycin and ofloxacin in pure mixture and in pharmaceutical binary dosage forms. A new concept of area under curve (AUC) is proposed for simultaneous estimation of two drugs by these methods. Method A involves the use of DDQ (2,3-dichloro-5,6-dicyano-1,4-benzoquinone) as analytical reagent and the AUC between 390nm and 690nm for DDQ was used for determination. Method B involves the use of p-CA (p-chloranilic acid: 2,5-dichloro-3,6-dihydroxy-1,4-benzoquinone) as an analytical reagent and the AUC between 400nm and 700nm for p-CA was used for determination. The methods developed and construction of calibration curves using two analytical reagents viz., DDQ and p-CA are described. Optical and analytical parameters for the individual and simultaneous determination of azithromycin and ofloxacin using AUC are tabulated. These methods have been validated and compared with HPLC methods in terms of standard deviation, t-test and F-test.
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Spectrophotometry, Simultaneous assay, AUC, Azithromycin, Ofloxacin
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