D-Mannose-Decorated Chitosan Nanoparticles for Enhanced Targeting of 5-Fluorouracil in the Therapy of Colon Cancer
DOI:
https://doi.org/10.37285/ijpsn.2021.14.1.7Abstract
Colorectal cancer is one of the most familiar malignant tumor, which requires an efficient system specially fabricated for targeted delivery of anticancer agents. 5-Fluorouracil (5-FU) is mostly used as an antineoplastic agent in gastrointestinal cancer. Ligand-based targeting approaches increase the internalization of NPs within resistant cells, which results in highly well-organized treatment, markedly reduces systemic toxicities, and minimize collateral damage to adjacent healthy cells. Mannose-conjugation was carried out to increase its uptake by the cancer cells through receptor-mediated endocytosis. It was attempted to develop d-mannose conjugated 5-fluorouracil (5-FU) entrapped nanoparticles (NPs) with an objective to target colon cancer. The conjugated-NPs were characterized for distinct parameters, for instance, pH, viscosity, morphology of particles, particle size (PS), size-distribution, polydispersity index, %yield, surface charge, and entrapment efficiency. However, formulations were also examined (in vitro) for cell cytotoxicity against CT26 and drug leakage in dissolution fluid. The fabricated NPs produced sustained drug release effect and exhibited release of 5-FU for 24 h. In addition, conjugation of d-mannose with NPs significantly augmented sustained effect over non-conjugated formulations. The preliminary results obtained for this study suggested that conjugation of d-mannose with chitosan NPs augmented the targeting effect in colon cancer. In conclusion, d-mannose conjugated NPs proved to be a more efficient carrier system over other conventional formulation as a delivery system for tumor.
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d-mannose, chitosan, 5-fluorouracil, nanoparticles, CT26, colon cancer
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