An In Vitro Study on Inhibiting Pathogenic Activity by Biosynthesized Silver Nanoparticles using Dioscorea Pentaphylla
DOI:
https://doi.org/10.37285/ijpsn.2021.14.2.7Abstract
Development of biologically stimulated experimental processes for the biosynthesis of nanoparticles is an essential branch of nanobiotechnology. Biocompatible silver nanoparticles have acknowledged significant notice in recent years because of their capable applications in bioimaging, biosensors, biolabels, and biomedicine. This study reports a green chemistry approach for the biological synthesis of silver nanoparticles by using the rhizomes extract of Dioscorea Pentaphylla. The properties of synthesis nanoparticles were characterized by UV-Visible spectroscopy, FT-IR, SEM, TEM and EDS. Surface Plasmon Resonance (SPR)
spectrum for silver nano-particles is obtained at 426 with dark brown color. FT-IR spectroscopy revealed that silver nanoparticles were functionalized with bio- molecules that have primary amine group (NH2), carbonyl phenolic group (-OH) and other stabilizing functional groups. The Morphological studies revealed that the particles are spherical in shape and in the size ranged from 20 ± 1nm. The effects of silver nanoparticles concentration and extract concentration were investigated and DPR-AgNPs showed the better pharma- cological applications.
Downloads
Metrics
Keywords:
Dioscorea Pentaphylla, Bioreduction, UV-Visible Spectroscopy, Anti-inflammatory activity, Antibacterial activity
Downloads
Published
How to Cite
Issue
Section
References
Shahverdi AR, Sara Minaeian, Hamid Reza Shahverdi, Hossein Jamalifar and Ashraf-Asadat Nohi (2007). Rapid synthesis of silver nanoparticles using culture supernatants of Entero- bacteria: A novel biological approach, Process Biochemistry, 42: 919–923
Akolade JO, Etuk-Udo G A, Dozie-Nwachukwu S O and Olalekan D O (2012). Phytosynthesis of silver nanoparticles and their application to bioactive products development, Environmental Research Journal, 6: 135-139
Mittal AK, Kaushik Thanki, Sanyog Jain and Uttam Chand Banerjee (2016). Comparative studies of anticancer and antimicrobial potential of bioinspired silver and silver-selenium nanoparticles, Appl. Nanomed, 1: 1-6
Masooleh AK, Asadollah Ahmadikhah and Abbas Saidi (2019). Green synthesis of stable silver nanoparticles by the main reduction component of green tea (Camellia sinensis L.), IET Nano-biotechnol., 13(2): 183-188
Anoop MV and Bindu A R (2015). In-vitro Anti-inflammatory Activity Studies on Syzygium zeylanicum (L.) DC Leaves, International Journal of Pharma Research & Review, 4:18-27
Singh AK and Nakate UT (2013). Microwave Synthesis, Characterization and photocatalytic properties of SnO2 nanoparticles, Advances in Nanoparticles, 2: 66-70
Avouris P (1995). Manipulation of matter at the atomic and molecular levels, Accounts Chem. Res, 28: 95-102
Dutta B (2015). Food and medicinal values of certain species of Dioscorea with special reference to Assam, Journal of Pharmacognosy and Phytochemistry, 3: 15-18
Bhakya S, Muthukrishnan S, Sukumaran M and Muthukumar M (2016). Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity, Appl Nanosci, 6:755–766.
Chhikara BS (2017). Current trends in nanomedicine and nanobiotechnology research, Appl. Nanomed, 2: 1-6
Jhanwar D and Sharma J (2016). Chemotherapeutic and chemopreventive effect of ZnO nanoparticles on DMBA/croton oil induced mice skin carcinogenesis, Appl. Nanomed, 1:7-11
Ajayi E and Afolayan A (2017).Green synthesis, characterization and biological activities of silver nanoparticles from alkalinized Cymbopogon citrates Stapf. Adv. Nat. Sci. Nanosci. Nanotechnol, 8 :1-8
Patel I, Priyanka Chaudhari, Anita Solanki and Dipika Rathod (2019). Flower-Mediated Phytosynthesis of Silver Nano-particles from Butea monosperma Lam. and their Evaluation for Antibacterial Activity, International Journal of Pharmaceutical Sciences and Nanotechnology, 12(6) MS ID: IJPSN-9-1-19- RATHOD,4718-4723
Sahni G, Amit Panwar and Balpreet Kaur (2015).Controlled green synthesis of silver nanoparticles by Allium cepa and Musa acuminata with strong antimicrobial activity, Int Nano Lett, 5: 93–100
Goud GK, Naveen Kumar Veldurthi, Vithal M and Gopal Reddy (2016). Characterization and evaluation of biological and photocatalytic activities of selenium nanoparticles synthesized using yeast fermented broth, Appl. Nanomed, 1:12-19
Hasanpoora M, Aliofkhazraeia M and Delavaria H (2015). Microwave-assisted synthesis of zinc oxide nanoparticles, Procedia Materials Science, 11: 320-325
Erjaee H, Hamid Rajaian and Saeed Nazifi (2017). Synthesis and characterization of novel silver nanoparticles using Chamaemelumnobile extract for antibacterial application, Adv. Nat. Sci. Nanosci. Nanotechnol, 8:1-9
Song JY, Hyeon-Kyeong Jang and Beom Soo Kim (2009). Biological synthesis of gold nanoparticles using Magnolia kobus and Diopyros kaki leaf extracts, Process Biochemistry, 44: 1133– 1138
Behera KK, Santilata Sahoo and Aratibala Prusti (2009). Relative Agronomic performance of different Dioscorea species found in different parts of Orissa, Nature and Science,7(3): 23-35
Kathiraven T, Sundaramanickam A, Shanmugam N and Balasubra- manian T (2015). Green synthesis of silver nanoparticles using marine algae Caulerpa racemosa and their antibacterial activity against some human pathogens, Appl Nanosci, 5:499–504
Kaviya S, Santhanalakshmi J, Viswanathan B, Muthumary J and Srinivasan K (2011). Biosynthesis of silver nanoparticles using citrus sinensis peel extract and its antibacterial activity, Spectrochimica Acta Part A, 79, pp. 594–598
Kim JS and Poulose EK (2007). Antimicrobial effects of silver nanoparticles, Nanomed. Nanotechnol. Biol. Med., 3(1): 95–101
Ejaz K, Haleema Sadia, Ghazna Zia, Shabnam Nazir, Abida Raza, Shaukat Ali, Tariq Iqbal and SaiqaAndleeb (2018). Biofilm reduction, cell proliferation, anthelmintic and cytotoxicity effect of green synthesised silver nanoparticle using Artemisia vulgaris extract, IET Nanobiotechnol., 12(1): 71-77
Valodkar M, Ravirajsinh N. Jadeja, Menaka C. Thounaojam, Ranjitsinh V. Devkar and Sonal Thakore (2011). In vitro toxicity study of plant latex capped silver nanoparticles in human lung carcinoma cells, Materials Science and Engineering C,8: 1723- 1728
Jehan M, Fiza Saeed, Zanib Khan, Muddassar Shah, Ayesha Sikandar, Afia Inayat, Sartaj Ali, Arshad Mehmood Khan, Ayesha Talib, Muhammad Aasim and Abid Ali Khan (2018). Investigative evaluation of Cassia absusfor antibacterial capacity and biomimetic synthesis of silver nanoparticles, IET Nanobiotechnol., 2(8): 1062-1066
Mohammadinejad R, Sh. Pourseyedi, Baghizadeh A and Sh. Ranjbar, Mansoori G A (2013). Synthesis of Silver Nanoparticles Using Silybum Marianum Seed Extract, Int. J. Nanosci. Nanotechnol, 9: 221-226
Kumari N, Neha Sinha, Akruti and K.K (2019). Gupta Efficacy of Silver Nanoparticles Synthesized fromHymenocallis species (Spider Lilly) Leaf Extract asAntimicrobial Agents, Inter- national Journal of Pharmaceutical Sciences and Nano- technology, 12(5): 4644-4647, MS ID: IJPSN-7-1-19-GUPTA,
Sahu N, Deepika Soni, Chandrashekhar B, Satpute D B, Sivanesan Saravanadevi, Sarangi BK and Pandey R A (2016). Synthesis of silver nanoparticles using flavonoids: hesperidin, naringin and diosmin, and their antibacterial effects and cytotoxicity, Int Nano Lett, 6: 173–181
Sheikh N, Yogendra Kumar, Misra A K and Lokho Pfoze (2013). Phytochemical screening to validate the ethnobotanical importance of root tubers of Dioscorea species of Meghalaya, North East India’, Journal of Medicinal Plants Studies, 1: 62-69
Prakash G and Hosetti B B (2012). Bio-efficacy of Dioscorea pentaphylla from Midmid-Western Ghats, India, Toxicology International, 19:100-105
Kumar PK, Willi Paul and Chandra P. Sharma (2011). Green synthesis of gold nanoparticles with Zingiber officinale extract: Characterization and blood compatibility, Process biochemistry, 46: 2007-2013
Tran QH, Van Quy Nguyen and Anh-Tuan Le (2013). Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives, Adv. Nat. Sci.: Nanosci. Nanotechnol, 4: 1-20
Singh R, Tasleem Arif, Imran Khan and Poonam Sharma (2014). Phytochemicals in antidiabetic drug discovery, J. Biomed. Ther. Sci.1:1-33
Das R, Siddarth S. Nath, Dipankar Chakdarb, Gautam Gope and Ramendhu Bhattacharjee (2010). Synthesis of silver nanoparticles and their optical properties’Journal of Experimental Nanoscience, 5: 357–362
Roy K, Sarkar C K and Ghosh C K (2015). Apium Graveolens Leaf Extract-Mediated Synthesis of Silver Nanoparticles And Its Activity On Pathogenic Fungi’. Digest Journal of Nanomaterials and Biostructures, 10: 393 – 400
Roy K, Sarkar C K and Ghosh C K (2015). Single-Step Novel Biosynthesis of Silver nanoparticles using Cucumis Sativus Fruit Extract and Study of its Photcatalytic and Antibacterial Activity’, Digest Journal of Nanomaterials and Biostructures, 10:107 – 115
Sinha SN, Dipak Paul, Nilu Halder, Dipta Sengupta and Samir Kumar Patra (2015). Green synthesis of silver nanoparticles using fresh water green alga Pithophora oedogonia (Mont.) Wittrock and evaluation of their antibacterial activity, Appl Nanosci,5: 703–709
Sau TK, Rogach A L, Jäckel F, Klar T A and Feldmann J (2010). Properties and applications of colloidal nonspherical noble metal nanoparticles, Adv. Mat, 22:1805-1825.
Shanmugam N, Rajkamal P, Cholan S, Kannadasan N, Sathishkumar K, Viruthagiri G, and Sundaramanickam A (2014). Biosynthesis of silver nanoparticles from the marine seaweed Sargassum wightii and their antibacterial activity against some human pathogens, Appl Nanosci, 4: 881–888
Sheny DS, Joseph Mathew and Daizy Philip (2011). Phytosynthesis of Au, Ag and Au–Ag bimetallic nanoparticles using aqueous extract and dried leaf of Anacardium occidentale’, Spectro- chimica Acta Part A: Molecular and Biomolecular Spectro-scopy, 79: 254–262
Paul S, Changam Sheela Sasikumar and Anita RJ singh (2014). Synthesis of silver nanoparticles by the green reagents (Polyphenolic Compounds) Prepared From Ganoderma, Lucidum, Phellinus Igniarius and Parmelia Perlata, International Journal Of Pharmaceutical and Chemical Sciences, 3: 939-947
Layeghi-Ghalehsoukhteh S, JafarJalaei, Mehdi Fazeli, Parastoo Memarian and Seyed Shahram Shekarforoush (2018). Evaluation of ‘green’ synthesis and biological activity of gold nanoparticles using Tragopogon dubius leaf extract as an antibacterial agent, IET Nanobiotechnol., 2(8): 1118-1124
Ghosh S, Vijay Singh Parihar, Piyush More, Dilip D Dhavale and Balu A Chopade (2015). Phytochemistry and Therapeutic Potential of Medicinal Plant: Dioscorea bulbifera’, Med chem., 5: 160-172.
