HPTLC Fingerprint Profile of Leaf and Stem Methanolic Extract of Asclepias Curassavica L.

Abstract

Asclepias curassavica, a member of the family Asclepiadaceae, is useful for a variety of health conditions such as abdominal pain, hemorrhage, asthma, and piles. It has a wide range of chemical constituents including flavonols, flavonol glycosides, triterpenes, cardenolides and many other biologically active compounds. Here we attempted to characterize the HPTLC profile for Leaf and Stem of methanolic extracts of Asclepias Curassavica L. Asclepias Curassavica L. family Asclepiadaceae, is important medicinal plant and traditionally used in indigenous system of medicine Extract of Asclepias Curassavica L. Methanolic extracts were prepared using sonication. The mobile phase for triterpenes and saponins was used in different ratio for better separation. The fingerprint showed better resolution after derivatization in methanolic sulphuric acid reagent. The fingerprint has revealed a distinct and repetitive pattern of bands. In conclusions, the HPTLC profile of the plant will be helpful for crude drug identification, serve to check the adulteration and act as biomarker for this plant in the Pharmaceutical industry.