Comparative Evaluation of Triclosan in Selected Anti-bacterial Soaps

DOI:

https://doi.org/10.37285/ijpsn.2024.17.1.2

Authors

  • Nija B Department of pharmaceutical chemistry, Al Shifa College of Pharmacy, Perinthalmanna, Malappuram
  • Fathima Rahna K Department of pharmaceutical chemistry, Al Shifa College of Pharmacy, Perinthalmanna, Malappuram
  • Jahana Sherin Department of pharmaceutical chemistry, Al Shifa College of Pharmacy, Perinthalmanna, Malappuram
  • Naslin Department of pharmaceutical chemistry, Al Shifa College of Pharmacy, Perinthalmanna, Malappuram
  • Prasanth S S Department of pharmaceutical chemistry, Al Shifa College of Pharmacy, Perinthalmanna, Malappuram
  • Shebina P Rasheed Department of pharmaceutical chemistry, Al Shifa College of Pharmacy, Perinthalmanna, Malappuram
  • Jemi Jacob Department of pharmaceutical chemistry, Al Shifa College of Pharmacy, Perinthalmanna, Malappuram
  • Neethu Varghese Department of pharmaceutical chemistry, Al Shifa College of Pharmacy, Perinthalmanna, Malappuram
  • Arun Rasheed Department of pharmaceutical chemistry, Al Shifa College of Pharmacy, Perinthalmanna, Malappuram

Abstract

Background of the topic: Triclosan is a widely used antimicrobial agent in personal care products. Triclosan is readily absorbed into the skin and present in the human tissues and fluids.   But the triclosan showed many side effects including carcinoma. Therefore the determination of triclosan in various personal health care products by simplest analytical methods is very much significant. 

Purpose of the study: The present study was aimed to do the comparative evaluation of triclosan in selected soaps and the evaluation was carried out by using ultraviolet spectroscopy.

Methods: Comparative evaluation of the selected four anti-bacterial soaps were carried out and the percentage of triclosan was determined by calibration curve method. The analytical technique used was ultra-violet spectroscopy by using the solvent ethanol. The Linearity was tested using the standard triclosan in the range of 300 µg/ml - 1500 µg/ml.   The validation methods such as ruggedness, repeatability, intermediate precision, linearity were done for all the four selected samples. Accurately weighed soap samples and dissolved in 3 ml of water. Transferred this solution into a separating funnel and extracted with chloroform. Organic layer was separated out and pipette out 1 ml of this organic layer and diluted to 10 ml with ethanol. The absorbance of the sample solution was measured at 307 nm.

Results: Comparative evaluation of the selected anti bacterial soaps and triclosan content was estimated by UV-spectroscopy. The λmax was found to be 307nm. The method was found to be linear within 300 µg/ml - 1500 µg/ml.  The percentage of triclosan present in selected four samples A, B, C and D were 0.28±0.8772, 0.24±0.9878, 0.32±1.123 and 0.73±0.9932 respectively. 

Conclusions: The percentage of triclosan in sample A sample B were within the limit and sample C and sample D slightly higher than that of the safe limit. The method used for the estimation of triclosan was UV spectroscopy method which was validated using ICH guidelines.

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Keywords:

Triclosan, Antibacterial soaps, ultraviolet spectrophotometry

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Published

2024-02-01

How to Cite

1.
B N, Rahna K F, Sherin J, Naslin, S S P, Rasheed SP, et al. Comparative Evaluation of Triclosan in Selected Anti-bacterial Soaps. Scopus Indexed [Internet]. 2024 Feb. 1 [cited 2024 Nov. 20];17(1):7119-23. Available from: https://ijpsnonline.com/index.php/ijpsn/article/view/2883

Issue

Vol. 17 No. 1 (2024): January-February 2024

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC

References

Jones RD, Jampani HB, Newman JL, Lee AS. Triclosan: a review of effectiveness and safety in health care settings. Am J Infect Control. 2000 Apr;28(2):184-96.

Yueh MF, Tukey RH. Triclosan: A Widespread Environmental Toxicant with Many Biological Effects. Annu Rev Pharmacol Toxicol. 2016;56:251-72.

Milanovic M, Duric L, Milosevic N, Milic N. Comprehensive insight into triclosan-from widespread occurrence to health outcomes. Environ Sci Pollut Res Int. 2021 Nov 6:1–22.

Sinicropi MS, Iacopetta D, Ceramella J, Catalano A, Mariconda A, Pellegrino M, Saturnino C, Longo P, Aquaro S. Triclosan: A Small Molecule with Controversial Roles. Antibiotics (Basel). 2022 May 30;11(6):735.

Lee JD, Lee JY, Kwack SJ, Shin CY, Jang HJ, Kim HY, Kim MK, Seo DW, Lee BM, Kim KB. Risk Assessment of Triclosan, a Cosmetic Preservative. Toxicol Res. 2019 Apr;35(2):137-154.

Du L, Li M, Jin Y. Determination of triclosan in antiperspirant gels by first-order derivative spectrophotometry. Pharmazie. 2011 Oct;66(10):740-3.

Lunardi N, Correr AB, Rastelli AN, Lima DA, Consani RL. Spectrophotometric evaluation of dental bleaching under orthodontic bracket in enamel and dentin. J Clin Exp Dent. 2014 Oct 1;6(4):e321

Vered Y, Zini A, Mann J, DeVizio W, Stewart B, Zhang YP, Garcia L. Comparison of a dentifrice containing 0.243% sodium fluoride, 0.3% triclosan, and 2.0% copolymer in a silica base, and a dentifrice containing 0.243% sodium fluoride in a silica base: a three-year clinical trial of root caries and dental crowns among adults. J Clin Dent. 2009;20(2):62-5.

Mahitha P, Gurupadayya BM and Chandan RS: Analytical method development and validation of triclosan in dental formulations. Int J Pharm Sci & Res 2014; 5(9): 3810-16.

Piccoli A, Fiori J, Andrisano V, Orioli M. Determination of triclosan in personal health care products by liquid chromatography (HPLC). Farmaco. 2002 May;57(5):369-72.

Lu H, Ma H, Tao G. Spectrophotometric determination of triclosan in personal care products. Spectrochim Acta A Mol Biomol Spectrosc. 2009 Sep 1;73(5):854-7.

Kaur I, Gaba S, Kaur S, Kumar R, Chawla J. Spectrophotometric determination of triclosan based on diazotization reaction: response surface optimization using Box-Behnken design. Water Sci Technol. 2018 May;77(9-10):2204-2212.