Phytochemical Screening, Analgesic and Anti-Inflammatory Activity of the Ethanol Extract of the Cnidoscolus Phyllacanthus Leaves

Analgesic and Anti-Inflammatory Activity Cnidoscolus phyllacanthus Leaves

DOI:

https://doi.org/10.37285/ijpsn.2023.16.4.5

Authors

  • Dr. Gurdeep Singh Associate Professor, School of Pharmaceutical Sciences, Lovely Professional University, Phagwara, Punjab
  • Ritesh Patel Department of Pharmaceutical Chemistry, Institute of Pharmacy, Indore, Madhya Pradesh
  • Mukesh Kr. Singh School of Pharmaceutical Sciences, IFTM University, Moradabad, Uttar Pradesh
  • Gaurav Agarwal Department of Pharmaceutical Chemistry, Institute of Pharmacy, Indore, Madhya Pradesh

Abstract

Objective: The present study aimed to investigate the analgesic and anti-inflammatory activity of the Cnidoscolus phyllacanthus (family Euphorbiaceae) ethanolic leaves extract in the laboratory using in vivo methods to justify its traditional use in the above-mentioned pathological conditions.

Methods: Phytochemical screening was done to find the presence of various secondary metabolites of the plant. In vivo, analgesic activity was performed employing the acetic acid-induced writhing test and tail immersion test on Swiss albino mice at doses of 50, 100 and 200 mg/kg body weight. An anti-inflammatory activity test was done on Wistar rats at three doses (50, 100 and 200 mg/kg body weight) using a carrageenan-induced paw oedema test. Data were analyzed by one-way analysis of variance (ANOVA), and Dunnett's t-test was used as the significance test. P value was considered as the minimum level of significance. 

Results: Phytochemical screening revealed the presence of coumarin, flavonoids, monoterpenes, diterpenes and naphthoquinones in the extract. In the acetic acid-induced writhing test, the per cent inhibition of writhing response by the extract was 12.82%, 25.64% and 76.67% at 50, 100 and 200 mg/kg doses, respectively (p < 0.01) and showed an increase in pain threshold in tail immersion method. The extract significantly (p < 0.05 and p < 0.01) inhibited carrageenan-induced inflammatory response in rats in a dose-related manner. Per cent, inhibition in paw oedema was 16.39 %, 29.50 % and 52.45 % at 50, 100 and 200 mg/kg doses, respectively. 

Conclusion: The results obtained from the tests indicate that the plant might have one or more secondary metabolite(s) having central and peripheral analgesic and anti-inflammatory activity.

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Keywords:

Cnidoscolus phyllacanthus, Analgesic, Anti-inflammatory, Indomethacin, Tail immersion, Carrageenan

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Published

2023-07-31

How to Cite

1.
Dr. Gurdeep Singh, Ritesh Patel, Mukesh Kr. Singh, Gaurav Agarwal. Phytochemical Screening, Analgesic and Anti-Inflammatory Activity of the Ethanol Extract of the Cnidoscolus Phyllacanthus Leaves : Analgesic and Anti-Inflammatory Activity Cnidoscolus phyllacanthus Leaves . Scopus Indexed [Internet]. 2023 Jul. 31 [cited 2024 Nov. 4];16(4):6882-7. Available from: https://ijpsnonline.com/index.php/ijpsn/article/view/2961

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