Stability Indicating RP-HPLC Method For Simultaneous Estimation of Efonidipine Hydrochloride Ethanolate and Metoprolol Succinate

DOI:

https://doi.org/10.37285/ijpsn.2024.17.5.7

Authors

  • Ashok B Patel B. k. mody government pharmacy college, Rajkot
  • Shivangee B Pithadiya Department of Pharmaceutical Sciences, Faculty of Health Sciences, Marwadi University, Rajkot-Morbi Highway, Rajkot,
  • Rushaliben V Zala B. k. mody government pharmacy college, Rajkot, india.
  • Dhruvi U Parmar B. k. mody government pharmacy college, Rajkot, india
  • Ashvin V Dudhrejiya B. k. mody government pharmacy college, Rajkot
  • Amitkumar J Vyas B. k. mody government pharmacy college, Rajkot, india.
  • Ajay I Patel B. k. mody government pharmacy college, Rajkot, india.

Abstract

A simple, precise, robustness, and accurate Stability Indicating RP-HPLC method has been developed and validated for the simultaneous assay of Efonidipine hydrochloride Ethanolate and metoprolol succinate in synthetic mixture. Isocratic RP-HPLC method was developed on Zodiac CN, (250 mm × 4.6 mm, 5μ) column using mobile phase as 20 mM Ammonium acetate: ACN (40:60) at a flow rate of 1.0 ml/min and the detection was carried out at 225 nm using photodiode array detector. Forced degradation study was carried out by oxidation, hydrolysis, photolysis, and heating the drug. The method was found to be linear in the concentration range of 5µg/ml- 500 µg/ml with correlation coefficient of 0.999 for Efonidipine hydrochloride Ethanolate and 6.25–625

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Keywords:

Efonidipine hydrochloride Ethanolate, Metoprolol succinate, Stability indicating, RP-HPLC, ICH (Q2R1) guideline, force degradation

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Published

2024-10-15

How to Cite

1.
Patel AB, Pithadiya SB, Zala RV, Parmar DU, Dudhrejiya AV, Vyas AJ, et al. Stability Indicating RP-HPLC Method For Simultaneous Estimation of Efonidipine Hydrochloride Ethanolate and Metoprolol Succinate. Scopus Indexed [Internet]. 2024 Oct. 15 [cited 2024 Dec. 30];17(5):7605-13. Available from: https://ijpsnonline.com/index.php/ijpsn/article/view/2969

Issue

Vol. 17 No. 5 (2024): September-October 2024

Section

Research Articles
Crossref
Scopus
Google Scholar
Europe PMC

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