Antidiabetic Activity of Aqueous and Methanolic Extract of Dried Pericarp (Shell) of Juglans Regia L.
DOI:
https://doi.org/10.37285/ijpsn.2019.12.3.3Abstract
The aim of the present study was to investigate the in-vitro anti-diabetic activity of aqueous and methanolic extract of dried pericarp (Shell) of Juglans regia L. The preliminary qualitative phytochemical screening was also undertaken to reveal the presence of Phyto-constituents in the extracts. Phytochemical screening of aqueous extract shows the presence of polyphenols, flavanoids, cardiac glycosides, carbohydrates and reducing sugar while methanolic extract show the presence of alkaloids, polyphenols, cardiac glycosides, carbohydrates and reducing sugar. Pharmacological evaluation of Juglans regia was performed by α-amylase inhibition assay method. Percentage inhibition of α amylase at dose of 50mg/mL, 100 mg/mL and 150 mg/mL of aqueous and methanolic extract was found to be 33.33, 66.67, 93.34 and 32.27, 59.64, 90.74 respectively. Among the extracts the aqueous extract showed highest activity as compared to control and methanolic extract.
Downloads
Download data is not yet available.
Metrics
Metrics Loading ...
Keywords:
Juglans regia, Anti-diabetic, Cardiac glycosides, α-amylase, Polyphenols, Phytochemical
Downloads
Published
2019-05-31
How to Cite
1.
Agrawal KK, Agrawal N, Gupta J, Gupta T. Antidiabetic Activity of Aqueous and Methanolic Extract of Dried Pericarp (Shell) of Juglans Regia L. Scopus Indexed [Internet]. 2019 May 31 [cited 2024 Nov. 19];12(3):4531-3. Available from: https://ijpsnonline.com/index.php/ijpsn/article/view/299
Issue
Section
Research Articles
References
Delaviz H, Mohammadi J, Ghalamfarsa G, Mohammadi B, and Farhadi N (2017). A Review Study on Phytochemistry and Pharmacology Applications of Juglans Regia Plant. Pharmacogn Rev 11: 145-152.
Dzhafarova RE, Garaev GSh, and Dzhafarkulieva ZS (2009). Antidiabetic action of extract of Juglans regia L. Georgian Med News 170: 110-4.
Devi TN, Apraj V, Bhagwat A, Mallya R, Sawant L, and Pandita N (2011). Pharmacognostic and Phytochemical Investigation of Juglans regia Linn. Bark. Pharmacog J 3.
Eidi A, Moghadam JZ, Mortazavi P, Rezazadeh S, and Olamafar S (2013). Hepatoprotective effects of Juglans regia extract against CCl4-induced oxidative damage in Rats. Pharm Biol 51: 558-65.
Jahanbani R, Ghaffari SM, Salami M, Vahdati K, Sepehri H, Sarvestani NN, Sheibani N, and Moosavi-Movahedi AA (2016). Antioxidant and Anticancer Activities of Walnut (Juglans regia L.) Protein Hydrolysates Using Different Proteases. Plant Foods Hum Nutr 71: 402-409.
Labuckas DO, Maestri DM, Perelló M, Martínez ML, and Lamarque AL (2008). Phenolics from walnut (Juglans regia L.) kernels: Antioxidant activity and interactions with proteins. Food Chem 107: 607-612.
Mir MA, Sawhney SS, and Jassal MMS (2015). In-vitro antidiabetic studies of various extracts of Taraxacum officinale. The Pharma Inno J 4: 61-66.
Manikandan R, Anand AV and Muthumani GD (2013). Phytochemical and in vitro anti-diabetic activity of methanolic extract of Psidium guajava leaves. Int. J. Curr. Microbiol. App. Sci 2: 15-19.
Nair SS, Kavrekar V, and Mishra A (2013). Evaluation of In Vitro Anti diabetic Activity of Selected Plant Extracts. Int J Pharma Sci Inv 2: 12-19.
Narkhede MB, Ajimire PV, Wagh AE, Mohan M and Shivashanmugam AT (2011). In vitro antidiabetic activity of Caesalpina digyna (R.) methanol root extract. Asian J Plant Sci Res 1: 101-106.
Olokoba AB, Obateru OA, and Olokoba LB (2012). Type 2 Diabetes Mellitus: A Review of Current Trends. Oman Medi J 27: 269-273
Pitchaipillai R and Ponniah T (2016). In Vitro Antidiabetic Activity of Ethanolic Leaf Extract of Bruguiera Cylindrica L. – Glucose Uptake by Yeast Cells Method. IBBJ 2.
Ramprasad R and Madhusudhan S (2016). In vitro α-amylase and α-glucosidase inhibitory activities of ethanolic extract of Lactuca runcinata DC. Der Pharmacia Lettre 8: 231-236.
Rath BP and Pradhan D (2009). Antidepressant Activity of Juglans regia L. Fruit Extract. Int J Toxico & Pharmaco Res 1: 24-26.
Stalin C, Vivekanandan K and Bhavya E (2013). In-Vitro Antidiabetic Activity of Cardiospermum Halicacabum leaves Extracts. Glob J Medi Res XIII.
Sathiavelu A, Moorthy S, Archit SR, and Mythili S (2013). In Vitro anti-diabetic activity of aqueous extract of the medicinal plants Nigella sativa, Eugenia jambolana, Andrographis paniculata and Gymnema sylvestre. Int J Drug Dev & Res 5: 323-328.
Sattar NA, Hussain F, Iqbal T, and Sheikh MA (2012). Determination of in vitro antidiabetic effects of Zingiber officinale Roscoe. Braz J Pharma Sci 48.
Shah TI, Sharma E, and Ahmad G (2014). Juglans regia Linn: A Phytopharmacological Review. World J Pharm Sci 2: 364-373.
Vianna R, Brault A, Martineau LC, Couture R, Arnason JT, and Haddad PS (2011). In Vivo Anti-Diabetic Activity of the Ethanolic Crude Extract of Sorbus decora C.K. Schneid. (Rosacea): A Medicinal Plant Used by Canadian James Bay Cree Nations to Treat Symptoms Related to Diabetes. Evid Based Com Alt Med 2011.
Wianowska D, Garbaczewska S, Cieniecka-Roslonkiewicz A, Dawidowicz AL, and Jankowska A (2016). Comparison of antifungal activity of extracts from different Juglans regia cultivars and juglone. Microb Pathog 100: 263-267.
Zakavi F, Hagh LG, Daraeighadikolaei A, Sheikh AF, and Shooshtari ZL (2013). Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria. Int J Dent 2013.
Dzhafarova RE, Garaev GSh, and Dzhafarkulieva ZS (2009). Antidiabetic action of extract of Juglans regia L. Georgian Med News 170: 110-4.
Devi TN, Apraj V, Bhagwat A, Mallya R, Sawant L, and Pandita N (2011). Pharmacognostic and Phytochemical Investigation of Juglans regia Linn. Bark. Pharmacog J 3.
Eidi A, Moghadam JZ, Mortazavi P, Rezazadeh S, and Olamafar S (2013). Hepatoprotective effects of Juglans regia extract against CCl4-induced oxidative damage in Rats. Pharm Biol 51: 558-65.
Jahanbani R, Ghaffari SM, Salami M, Vahdati K, Sepehri H, Sarvestani NN, Sheibani N, and Moosavi-Movahedi AA (2016). Antioxidant and Anticancer Activities of Walnut (Juglans regia L.) Protein Hydrolysates Using Different Proteases. Plant Foods Hum Nutr 71: 402-409.
Labuckas DO, Maestri DM, Perelló M, Martínez ML, and Lamarque AL (2008). Phenolics from walnut (Juglans regia L.) kernels: Antioxidant activity and interactions with proteins. Food Chem 107: 607-612.
Mir MA, Sawhney SS, and Jassal MMS (2015). In-vitro antidiabetic studies of various extracts of Taraxacum officinale. The Pharma Inno J 4: 61-66.
Manikandan R, Anand AV and Muthumani GD (2013). Phytochemical and in vitro anti-diabetic activity of methanolic extract of Psidium guajava leaves. Int. J. Curr. Microbiol. App. Sci 2: 15-19.
Nair SS, Kavrekar V, and Mishra A (2013). Evaluation of In Vitro Anti diabetic Activity of Selected Plant Extracts. Int J Pharma Sci Inv 2: 12-19.
Narkhede MB, Ajimire PV, Wagh AE, Mohan M and Shivashanmugam AT (2011). In vitro antidiabetic activity of Caesalpina digyna (R.) methanol root extract. Asian J Plant Sci Res 1: 101-106.
Olokoba AB, Obateru OA, and Olokoba LB (2012). Type 2 Diabetes Mellitus: A Review of Current Trends. Oman Medi J 27: 269-273
Pitchaipillai R and Ponniah T (2016). In Vitro Antidiabetic Activity of Ethanolic Leaf Extract of Bruguiera Cylindrica L. – Glucose Uptake by Yeast Cells Method. IBBJ 2.
Ramprasad R and Madhusudhan S (2016). In vitro α-amylase and α-glucosidase inhibitory activities of ethanolic extract of Lactuca runcinata DC. Der Pharmacia Lettre 8: 231-236.
Rath BP and Pradhan D (2009). Antidepressant Activity of Juglans regia L. Fruit Extract. Int J Toxico & Pharmaco Res 1: 24-26.
Stalin C, Vivekanandan K and Bhavya E (2013). In-Vitro Antidiabetic Activity of Cardiospermum Halicacabum leaves Extracts. Glob J Medi Res XIII.
Sathiavelu A, Moorthy S, Archit SR, and Mythili S (2013). In Vitro anti-diabetic activity of aqueous extract of the medicinal plants Nigella sativa, Eugenia jambolana, Andrographis paniculata and Gymnema sylvestre. Int J Drug Dev & Res 5: 323-328.
Sattar NA, Hussain F, Iqbal T, and Sheikh MA (2012). Determination of in vitro antidiabetic effects of Zingiber officinale Roscoe. Braz J Pharma Sci 48.
Shah TI, Sharma E, and Ahmad G (2014). Juglans regia Linn: A Phytopharmacological Review. World J Pharm Sci 2: 364-373.
Vianna R, Brault A, Martineau LC, Couture R, Arnason JT, and Haddad PS (2011). In Vivo Anti-Diabetic Activity of the Ethanolic Crude Extract of Sorbus decora C.K. Schneid. (Rosacea): A Medicinal Plant Used by Canadian James Bay Cree Nations to Treat Symptoms Related to Diabetes. Evid Based Com Alt Med 2011.
Wianowska D, Garbaczewska S, Cieniecka-Roslonkiewicz A, Dawidowicz AL, and Jankowska A (2016). Comparison of antifungal activity of extracts from different Juglans regia cultivars and juglone. Microb Pathog 100: 263-267.
Zakavi F, Hagh LG, Daraeighadikolaei A, Sheikh AF, and Shooshtari ZL (2013). Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria. Int J Dent 2013.
