Analytical Methods for the Estimation of Levothyroxine and Levothyroxine Sodium in Pharmaceutical Formulations – A Review

DOI:

https://doi.org/10.37285/ijpsn.2023.16.6.10

Authors

  • Preethi G Department of Pharmaceutical Analysis,  Chalapathi Institute of Pharmaceutical Sciences,  Guntur
  • Siva Prasad M. Department of pharmaceutics,  Chalapathi Institute of Pharmaceutical Sciences
  • Prachet P. Department of Pharmaceutical Analysis,  Chalapathi Institute of Pharmaceutical Sciences,  Guntur
  • Rama Rao N. Department of pharmaceutics,  Chalapathi Institute of Pharmaceutical Sciences,Guntur

Abstract

Levothyroxine (L-T4) is used in the treatment of hypothyroidism and is a synthetic levoisomer of the thyroid hormone thyroxine produced by the thyroid gland. Levothyroxine sodium (L-T4S) salt has a high clinical utility. There are numerous studies that have been published that describe analytical methods for the estimation of L-T4 and L-T4S in pharmaceutical formulations and also in various biological matrices like blood, plasma, serum, and urine. This review article focused on analytical methods for estimating L-T4 and L-T4S in pharmaceutical formulations such as Thin Layer Chromatography (TLC), UV-visible spectrophotometer, High-Performance Liquid Chromatography (HPLC), Capillary electrophoresis, Luminescence, Sequential Injection Analysis (SIA), Voltammetry, and Hyphenated techniques. All the analytical methods for the estimation of L-T4 and L-T4S are given in tabular form. Out of all the analytical methods reported, HPLC is the most commonly used method for the estimation of L-T4 and L-T4S with optimum separation and resolution.

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Keywords:

L-T4, L-T4S, Hypothyroidism, Pharmaceutical, Analytical methods

Published

2023-11-15

How to Cite

1.
G P, M. SP, P. P, N. RR. Analytical Methods for the Estimation of Levothyroxine and Levothyroxine Sodium in Pharmaceutical Formulations – A Review. Scopus Indexed [Internet]. 2023 Nov. 15 [cited 2024 Apr. 20];16(6):7104-13. Available from: https://ijpsnonline.com/index.php/ijpsn/article/view/3196

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