Analytical Methods for the Estimation of Levothyroxine and Levothyroxine Sodium in Pharmaceutical Formulations – A Review
DOI:
https://doi.org/10.37285/ijpsn.2023.16.6.10Abstract
Levothyroxine (L-T4) is used in the treatment of hypothyroidism and is a synthetic levoisomer of the thyroid hormone thyroxine produced by the thyroid gland. Levothyroxine sodium (L-T4S) salt has a high clinical utility. There are numerous studies that have been published that describe analytical methods for the estimation of L-T4 and L-T4S in pharmaceutical formulations and also in various biological matrices like blood, plasma, serum, and urine. This review article focused on analytical methods for estimating L-T4 and L-T4S in pharmaceutical formulations such as Thin Layer Chromatography (TLC), UV-visible spectrophotometer, High-Performance Liquid Chromatography (HPLC), Capillary electrophoresis, Luminescence, Sequential Injection Analysis (SIA), Voltammetry, and Hyphenated techniques. All the analytical methods for the estimation of L-T4 and L-T4S are given in tabular form. Out of all the analytical methods reported, HPLC is the most commonly used method for the estimation of L-T4 and L-T4S with optimum separation and resolution.
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L-T4, L-T4S, Hypothyroidism, Pharmaceutical, Analytical methodsPublished
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