Ecofriendly Green Synthesis and Characterisation of Silver Nanoparticles of Hyoscyamus Niger Seeds and Evaluation of its Anthelmintic Activity

DOI:

https://doi.org/10.37285/ijpsn.2023.16.5.2

Authors

  • Fouzia Tehseen a:1:{s:5:"en_US";s:18:"Osmania university";}
  • Syed Safiullah Ghori
  • Mohammed Awais
  • Mohammed Hisamuddin
  • Syeda Lubna Eram
  • Ayesha Tasneen Hafsa

Abstract

Introduction: Nanotechnology is the design, characterisation and production of molecules or structures at nanometer scale. Metallic nanoparticles have been known for their therapeutic applications for thousands of years. Hyoscyamus niger is a traditional plant used as analgesic, sedative, anthelmintic, antitumour and febrifuge.

Objective: An eco-friendly approach was made for the synthesis of silver nanoparticles using aqueos seed extract of Hyoscyamus niger (H.n-AgNPs) and checked for possible anthelmintic activity.

Method: Preparation of silver nanoparticles in 1 mM aqueous silver nitrate from Hyoscyamus niger seed. The color shift from yellow to brownish red provided visible evidence of nanoparticle production. UV visible, Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray diffraction (XRD) examinations all validated it further. We tested the in vitro anthelmintic activity of various doses of H.n-AgNPs on Indian earthworms..

Results: The size of the H.n-AgNPs synthesized ranged between 8nm to183 nm. Synthesized NPs showed potential anthelmintic activity at 100µg/mlwith paralysis time and death time of 15 min and 34 min respectively where Albendazole was used as standard drug. The phytoconstituents like tannins and phenolic compounds may be responsible for precipitation of proteins or can attach with free proteins in gastrointestinal tract or glycoprotein on parasite cuticle and cause cell death. 

Conclusion: The H.n-AgNPs synthesized have potential anthelmintic activity which was concentration dependent. Further research will be done to design a new anthelmintic drug using H.n-AgNPs.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Keywords:

Nanotechnology, Hyoscyamus niger, characterisation, anthelmintic activity, phytoconstituents.

Published

2023-09-15

How to Cite

1.
Fouzia Tehseen, Syed Safiullah Ghori, Mohammed Awais, Mohammed Hisamuddin, Syeda Lubna Eram, Ayesha Tasneen Hafsa. Ecofriendly Green Synthesis and Characterisation of Silver Nanoparticles of Hyoscyamus Niger Seeds and Evaluation of its Anthelmintic Activity. Scopus Indexed [Internet]. 2023 Sep. 15 [cited 2024 Feb. 26];16(5):6945-52. Available from: https://ijpsnonline.com/index.php/ijpsn/article/view/3261

Issue

Section

Research Articles

References

Zhang D, Xin Lie MA, Yan Gu, Huang H, Zhang G. Green synthesis of Metallic Nanoparticles and their potential applications to treat cancer. Frontiers in chemistry, 2020; 8:799.

Singh J, Dutta T, Kim K, Rawat M, Samdar P and Kumar P. Green synthesis of metals and their oxide nanoparticles: applications for environmental remediation. Journal of Nanobiotechnology. 2018;16(84):1-24.

Lopez ES et al,. Metal based nanoparticles as Antimicrobial as antimicrobial agents: an Overview. Nanomaterials(Basel). 2020; 10(2):292.

Feng A, Wu S, Zhang H, Shao W and Xiao Z. Synthesis of silver nanoparticles with tunable morphologies via a reverse nano- emulsion route. Materials Transactions. 2013;54(8):1145-1148.

Santos Mm, QueirozMJ and Bapista PV. Enhancement of antibiotic effect via gold silver- alloy nanoparticles. Journal of Nanoparticle research. 2012; 14(5):859-866.

Aritonang HF, Onggo D, Ciptat C and Radiman CL. Insertion of platinum particles in bacterial cellulose membranes from PtCl4 and H2PtCl6 precursors. Macromolecular symposia. 2015; 353(1):55-56.

Raut RW, Haroon SM, Malaghe YS, Nikan BT and Kashid SB. Rapid biosynthesis of platinum and palladium metal nanoparticles using root extract of Asparagus racemosus Linn. Advanced materials Letters. 2013; 4(8):650-654.

Lok C, Ho C, Chen R, He Q, Yu W, Sun H, Tam PK, Chiu J, Che C. Silver nanoparticles -Partial oxidation and antibacterial activity. J. Biol. Inorg.Chem. 2007;12:527-534.

Shiraishi Y, Toshima N. Oxidation of ethylene catalysed by colloidal dispersions of poly(sodium acrylate)protected silver nanoclusters. Colloids and surfaces: A physiochem Eng Asp. 2000;169:59-66.

Chang LT, Yen CC.Studies on the preparation and properties of conductive polymers.VIII. Use of heat treatment to prepare metallized films from silver chelate of PVA and PAN. J Appl Polym Sci 1995; 55(2):371-374.

Sharverdi AR, Mianaeian S, Shahverdi HR, Jamalifar H, Nohi AA. Rapid synthesis of silver nanoparticles using culture supernatants of enterobacteria: a novel biological approach. Process Biochem. 2007; 42:919-923.

Rashid et al. Anthelmintic activity of silver extract nanoparticles synthesised from the combination of silver nanoparticles and M.charantia fruit extract. BMC complementary and applied medicine. 2016;16:242.

Balan K et al. Antidiabetic activity of silver nanoparticles from green synthesis using Lonicera japonica leaf extract. RSC Advanaces. 2016; 46.

Salve P et al. An Evaluation of Antimicrobial, Anticancer, Anti- inflammatory and Antioxidant activities of Silver Nanoparticles synthesized from leafextract of Madhuca londifolia utilizing Quantitative and Qualitative methods. Molecules 2022;27:6404.

Matejka P, Vlckova B, Vohlidal J, Pancoska P, Baumruk V. The role ot triton X-100 as an adsorbate and a molecular spacer on the surface of silver colloid: a surface enhanced Raman scaterring study. J Phys Chem 1992; 96(3):1361-1366.

Gengan R, Anand K, Phulukdaree A. A A549 lung cell line activity of biosynthesized silver nanoparticles using Albizia adianthifolia leaf. Colloids Surf., B. 2013;105:87-91.

Zaheer Z, Rafiuddin. Silver nanoparticles of self assembled films: Green synthesis and characterisation. Colloids surd Biointerfaces. 2012;90:48-52.

Siddiqui KS, Husen A and Rao RAK. A review on biosynthesis of silver nanoparticles and their biocidal properties. Journal of Nanobiotechnology. 2018;16(14).

Kumar V, Yadav SK. Plant mediated synthesis of Silver and gold nanoparticles and their applications. Journal of chemical technology and biotechnology. 2009; 84(2):151-157.

Li J et al. Chemical and pharmacological researches on Hyoscyamus niger. Chinese Herbal Medicines. 2011;3(2):117-126.

Aritonang HF, Koleangan H and Wuntu AD. Synthesis of Silver nanoparticles using Aqueous extract of medicinal plants and analysis of Antimicrobial activity. International Journal of microbiology. 2019.

Garima S, Riju B, Kunal K, Ashish RS and Rajendra PS. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. Journal of Nanoparticles Research. 2011;13(7):2988-2011.

Zhang XF et al., Silver nanoparticles: Synthesis, Characterisation, Properties, applications and therapeutic approaches. International Journals of Molecular science. 2016;17:1534.

Ajaiyeoba EO et al., Invitro anthelmintic activity of Buccholzia coriaceae and Gynandropsis gynandra extracts. Pharmaceutical Biology. 2001; 39(3):217-220.

Park EK et al., Silver nanoparticles incude cytotoxicity by a trojan -horse type mechanism. Toxicol. Vir. 2010;24:872-878.

Jena J, Pradhan N, Dash BP, Sukla LB, Panda P.Biosynthesis and characterization of silver nanoparticles using microalga Chlorococcumhumicola and its antibacterial activity. Int J Nanomater Biostruct. 2013;3:1-3.

Hamouda RA, Hissein MH, Abo -Elmagd RA, Bawazir SS. Syntheis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica. Scientific reports. 2019;9(13071).

Sastry M, Patil V, Sainkar Sr. Electrostatically controlled diffusion of carboxylic acid derivatized silver colloidal particles in thermally evaporated fatty amine films. J. Phys.Chem. 1998;109:1404-1410.

Vanden Bout DA. Metal nanoparticles: synthesis, characterization and applications. JAM Chem Soc. 2002;124:7874-75.

Cheng KM, Hung YW, Chen CC, Liu CC and Yound JJ. Green synthesis of chondroitin sulfate-capped silver nanoparticles: Characterization and surface modification. CarbohydrPolym. 2014;110:195-202.

Huang Q, Li D, Sun Y, Lu Y, Su X, Yang H, Wang W, Shao N, Hong J, Chen c. Biosynthesis of silver and gold nanoparticles by novel sundries Cinnamomum camphora leaf. Nanotechnol. 2007; 18:104-105.

Lin PC, Lin S, Wang PC, Sridhar R. Techniques for physiochemical characterization of nanomaterials. Biotechnol Adv. 2014;32:711-726.

Kanwal Z, Raza MA, Riaz S, Manzoor S, Tayyed A, Sajid I, Naseem S. Synthesis and characterization of silver nanoparticle-decorated cobalt nanocomposites and their density dependant antibacterial activity. R. Soc. Open Sci. 2019;6.

Ajitha B, Reddy AKY, Reddy PS. Biogenic nano-scale silver particles by Tephrosia purpurea leaf extract and their in born antimicrobial activity. Spectrochim Acta Part A. 2014;121:164-172.

Basavegowda N, Idhayadhulla A, Lee YR. Preparation of Au and Ag nanoparticles using Artemesia annua and their in vitro antibacterial and tyrosinase inhibitory activities. Mater sci eng C. 2014;43:56-64.

Bindhu MR, Umadevi M. Synthesis of monodispersed silver nanoparticles using Hibiscus cannabinus leaf extract and its antimicrobial activity. Spectrochim acta Part A. 2013;101:184-190.