Antioxidant Activity of Hydroalcoholic Extract of Labisa Pumila var. Alata, an Indigenous Plant of Malaysia

DOI:

https://doi.org/10.37285/ijpsn.2011.4.2.8

Authors

  • Christapher Parayil Varghese
  • Christina Ambrose
  • Veerasamy R
  • Pea Nai Hui
  • Chen Siang May

Abstract

 This study was carried out to investigate the antioxidant potential of crude extracts of Labisa pumila var. Alata, a small herbaceous under shrub widely available in Malaysia. In this study, antioxidant activity was evaluated by diphenyl picryl hydrazine (DPPH) free radical assay and FRAP assay methods. In the DPPH free radical assay, different concentrations of the extract (5, 10, 25, 50 and 75 μg/ml in ethanol) were prepared and mixed with DPPH ethanol solution. After incubation, a photometric assay was performed and values were plotted. The IC50 values were calculated by a linear regression of plots and compared with IC50 of ascorbic acid as the standard antioxidant. In the FRAP assay, different concentrations of extract (20, 40, 60, 80 and 100 μg/ml) were prepared and the reducing power was estimated by a photometric assay and values were plotted. The EC50 values were calculated by a linear regression of plots and compared with the EC50 of ascorbic acid as a standard reducing agent. The study revealed that the crude extract possesses antioxidant activity.

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Keywords:

Antioxidants, Labisa pumila;, free radical scavenging, reducing power;, DPPH, FRAP assay

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Published

2011-08-31

How to Cite

1.
Varghese CP, Ambrose C, R V, Hui PN, May CS. Antioxidant Activity of Hydroalcoholic Extract of Labisa Pumila var. Alata, an Indigenous Plant of Malaysia. Scopus Indexed [Internet]. 2011 Aug. 31 [cited 2024 Nov. 19];4(2):1418-22. Available from: https://ijpsnonline.com/index.php/ijpsn/article/view/430

Issue

Vol. 4 No. 2 (2011): July-September 2011

Section

Research Articles
Crossref
Scopus
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Europe PMC

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