Antidiabetic Activity of Isolated Compounds of Crinum solapurense Leaves Extract in Albino Wistar Rats

DOI:

https://doi.org/10.37285/ijpsn.2024.17.2.6

Authors

  • Sunayana Mali IES Institute of Pharmacy, IES University, Bhopal-462044, Madhya Pradesh, India
  • Anup Kumar Chakraborty IES Institute of Pharmacy, IES University, Bhopal
  • Reenu Yadav IES Institute of Pharmacy, IES University, Bhopal-462044, Madhya Pradesh, India
  • Jyotiram Sawale IES Institute of Pharmacy, IES University, Bhopal-462044, Madhya Pradesh, India

Abstract

Background: The leaves extracts of Crinum solapurense are examined for in-vivo antidiabetic pharmacological action.

Methods: Plant material including different multi-component mixes has a large number of bioactive chemicals. Therefore, it is crucial to separate and determine these compounds which have active phytoconstituents are responsible for the pharmacological action. The extracts for action in-vivo antidiabetic investigation from the leaves extract of Crinum solapurense are the subject of the current research project. From the leaves, ethanolic extracts of the medicinal plant Crinum solapurense, two alkaloid compounds—gulonic acid & shikimic acid—were identified. The alkaloids found in the phytoconstituents of Crinum solapurense leaves may be the cause of their pharmacological action.

Results: In the DNSA inhibition assay method the IC50 value was observed to be 5.31 & 11.17 μg/ml for shikimic acid & gulonic acid correspondingly. In the Iodine-Starch Technique the IC50 value was observed to be 9.91 & 12.70 μg/ml for shikimic acid & gulonic acid correspondingly. In the In-vivo animal study of Gulonic acid in the group that was not given any treatment, diabetic rats displayed considerable weight loss at the end of the trial (p ˂0.001). When dexamethasone was administered, the diabetic group's blood glucose levels significantly increased in comparison to the control group (p is equal to 0.01). Two parameters were improved in a dosage-dependent way when mice were given glibenclamide or gulonic acid at a dose of 50.00 or 100.00 milligrams per kilogram body weight. Diabetes led to a substantial rise in HbA1c (p˂0.05) in the group of diabetics who were not receiving treatment.

Conclusion: Based on the in-vivo animal study it was concluded that the leaves extracts of Crinum solapurense show significant effects of antidiabetic pharmacological action in diabetes induced animals.

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Keywords:

Crinum solapurens, gulonic acid, shikimic acid, in-vivo antidiabetic

Published

2024-03-31

How to Cite

1.
Mali S, Chakraborty AK, Yadav R, Sawale J. Antidiabetic Activity of Isolated Compounds of Crinum solapurense Leaves Extract in Albino Wistar Rats. Scopus Indexed [Internet]. 2024 Mar. 31 [cited 2024 May 20];17(2):7250-5. Available from: https://ijpsnonline.com/index.php/ijpsn/article/view/4325

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Section

Research Articles

References

Jawaid T, Kumari N, Mehnaz K and Saud A. Evaluation of antidiabetic activity of polyherbal formulation “vasant kusumakar ras” on alloxan-induced and dexamethasone-induced diabetic rats. Journal of pharmaceutical research international. 2021 Nov 20; 33(51A): 13-22.

Tam H, Thanh N, Tram T. Isolation and characterization of actinobacteria in rhizosphere of crinum latifolium (L.) cultivated in Tay Ninh province, Vietnam. International journal of innovations in engineering and technology. 2019; 13(4): 026-033.

Gaikwad SP, Garad KU, Gore RD. Crinum solapurense (Amaryllidaceae), a new species from Maharashtra, India. Kew bulletin. 2014 May 4; 69: 9501-9505.

Rasul MG. Extraction, isolation & characterization of natural products from medicinal plants. International journal of basic sciences and applied computing. 2018 Dec; 2(6): 1-6.

Tegegne BA, Mekuria AB, Birru EM. Evaluation of anti-diabetic and anti-hyperlipidemic activities of hydro-alcoholic crude extract of the shoot tips of Crinum abyssinicum hochst. ex A. Rich (Amaryllidaceae) in Mice. Journal of experimental pharmacology. 2022 Feb 1: 4: 27-41.

Bjora CS, Kwembeya EG, Bogner J, Nordal I. Geophytes diverging in rivers-a study on the genus Crinum, with two new rheophytic taxa from Cameroon. Taxon. 2009 May; 58(2): 561–571.

Ghane SG, Attar UA, Yadav PB, Lekhak MM. Antioxidant, anti-diabetic, acetylcholinesterase inhibitory potential and estimation of alkaloids (lycorine and galanthamine) from Crinum species: An important source of anticancer and anti-Alzheimer drug. Industrial crops & products. 2018 Aug 30: 125: 168–177.

Sabiu S, Madende M, Ajao AA-N, Aladodo RA, Nurain IO, Ahmad JB. The genus allium (Amaryllidaceae: alloideae): features, phytoconstituents, and mechanisms of antidiabetic potential of allium cepa and allium sativum. In: Watson RR, Preedy VR, editors. Bioactive Food as Dietary Interventions for Diabetes. Academic Press. 2019; Second Edition: pp.137–154.

Ahmad MH, Singh V, Prasad RK. Preliminary phytochemical screening and antidiabetic activity of extracts of leaves of Crinum Latifolium Linn. Advance research journal of multidisciplinary discoveries. 2018 Jan 10; 32(7): 35–40.

Mvongo C, Mfopa A, Kamgang R, Oyono JL. Antidiabetic and antioxidant activities of crinum jagus extracts on induced diabetes rats MACAPOS 1. International journal of pharmacology, phytochemistry and ethnomedicine. 2016 Oct 19: 5: 86-95

Senbeta A, Awas T, Gure A. The qualitative and quantitative phytochemical investigation of Crinum species in Ethiopia. International journal of photochemistry and photobiology. 2019 July 2; 3(1):1.

Regassa R. Assessment of indigenous knowledge of medicinal plant practice and mode of service delivery in Hawassa city, southern Ethiopia. Journal of medicinal plants research. 2013 Mar 3; 7(9): 517–535.

Meresa A, Gemechu W, Basha H, Fekadu N, Teka F, Ashebir R and Tadele A. Herbal medicines for the management of diabetic mellitus in Ethiopia and Eritrea including their phytochemical constituents. American journal of advanced drug delivery. 2017 Apr 23; 5(01): 040–058.

Berihun A, Fekadu N, Geleta B. An ethno botanical review on medicinal plants used for the management of hypertension. Journal of clinical & experimental pharmacology. 2017 Feb 8; 7(2): 1–6.

Kloos H, Menberu T, Tadele A, Chanie T, Debebe Y, Abebe A, Zealiyas K, Tadele G, Mebruka M, Debella A. Traditional medicines sold by vendors in Merkato, Addis Ababa: aspects of their utilization, trade, and changes between 1973 and 2014. Ethiopian journal of health development. 2014; 28(2): 136-152.

Yineger H, Kelbessa E, Bekele T, Lulekal E. Plants used in traditional management of human ailments at Bale Mountains National Park, Southeastern Ethiopia. Journal of medicinal plants research. 2008 Jun 13; 2(6): 132–153.

Sebsebe D, Nordal I. Aloes and Lilies of Ethiopia and Eritrea. Addis Adaba, Ethiopia: Shama Books; 2010. Accessed 2022 Jan 20.

Teklehaymanot T, Giday M. Ethnobotanical study of medicinal plants used by people in Zegie Peninsula, Northwestern Ethiopia. Journal of ethnobiology and ethnomedicine. 2007 Mar 14; 3(1): 12.

Abebe W. An overview of Ethiopian traditional medicinal plants used for cancer treatment. European journal of medicinal plants. 2016 May 4; 14(4):1–16.

Mekuanent T, Zebene A, Solomon Z. Ethnobotanical study of medicinal plants in Chilga district, Northwestern Ethiopia. Journal of natural remedies. 2015 Dec; 15 (2):88–112.

Malviya V. Investigation of in-vitro antidiabetic study, antioxidant activity and anthelminthic property of various extracts of bitter cumin seeds: antidiabetic study, antioxidant activity, and anthelminthic property of bitter cumin seeds. International journal of pharmaceutical sciences and nanotechnology. 2023 July 31; 16(4): 6855-6874.