The Synthesis and Characterization of Mint Leaf (Mentha Spicata) Biological Silver Nanoparticles and their Impact on Rat’s Motor Coordination and Cognition
DOI:
https://doi.org/10.37285/ijpsn.2024.17.6.3Abstract
Background: The utilization of silver nanoparticles is rising, since nanotechnology is blossoming in daily products and biomedicine. According to research on animals, silver nanoparticles can damage the brain, liver, kidneys, intestines, and lungs, among other organs. Several studies have found a link between various methods of administering nanoparticles and cognitive and behavioral impairments.
Objective: The current study aims to investigate the impact of mint leaf silver nanoparticles on rats' psychological behavior.
Method: Silver nanoparticles were prepared using fresh leaves of mint as bioreducing agents. It was verified that silver nanoparticles were formed via characterization with UV spectroscopy, fluorescence spectroscopy, FTIR analysis, SEM, energy dispersive X-ray spectroscopy (EDX), and Zeta potential. Rats were treated with silver nanoparticles for 21 days orally (50 mg/kg body weight) and were tested for cognitive function of learning in the form of Morris water maze (MWM), nociception was studied by the Eddy hot plate test (HPT), Randal pain test (RPT), motor coordination was done by the Roto rod test (RRT), and depression-like behavior was studied with the Porsolt forced swim test (FWT).
Results: UV spectroscopy has revealed a peak at 420 nm indicating the formation of green silver nanoparticles; the fluorescent spectrum showed a peak at 400 nm displaying the fluorescent activity of synthesized silver nanoparticles. FTIR showed that silver nanoparticles were capped with mint leaf secondary metabolites. Even Zeta potential analysis confirmed the green nanoparticles; SEM has shown the spherical shape of silver nanoparticles; and X-rays with energy dispersive spectra reveal the constituent elements of silver generated at 3 keV. Learning and memory abilities of rats screened with MWM, HPT, RPT, RRT, and FWT. Data have revealed variations in test results between animals exposed to nanoparticles in comparison with control animals.
Conclusion: This study concludes that nanoparticles therapy in rats impairs learning, memory, and other behavioral abilities.
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Keywords:
UV spectroscopy, Fluorescent spectroscopy, FTIR, Scanning Electron Microscope, Morris water maze, Roto rod, depression.Published
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