Preparation, Characterization and In-vitro release of Piroxicam-loaded Solid Lipid Nanoparticles
DOI:
https://doi.org/10.37285/ijpsn.2010.3.3.12Abstract
Solid lipid nanoparticles (SLNs) of piroxicam where produced by solvent emulsification diffusion method in a solvent saturated system. The SLNs where composed of tripamitin lipid, polyvinyl alcohol (PVAL) stabilizer, and solvent ethyl acetate. All the formulation were subjected to particle size analysis, zeta potential, drug entrapment efficiency, percent drug loading determination and in-vitro release studies. The SLNs formed were nano-size range with maximum entrapment efficiency. Formulation with 435nm in particle size and 85% drug entrapment was subjected to scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for surface morphology, differential scanning calorimetry (DSC) for thermal analysis and short term stability studies. SEM and TEM confirm that the SLNs are nanometric size and circular in shape. The drug release behavior from SLNs suspension exhibited biphasic pattern with an initial burst and prolong release over 24 h.
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Keywords:
Solid lipid nanoparticles (SLNs), tripalmitin, polyvinyl alcohol, piroxicam, drug entrapment efficiency
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Published
2010-11-30
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1.
Verma VK, A R. Preparation, Characterization and In-vitro release of Piroxicam-loaded Solid Lipid Nanoparticles. Scopus Indexed [Internet]. 2010 Nov. 30 [cited 2024 Dec. 23];3(3):1136-4. Available from: https://ijpsnonline.com/index.php/ijpsn/article/view/530
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References
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Garcia-Fuentes M, Torres D, Alonso MJ. Design of lipid nanoparticles for the oral delivery of hydrophilic macromolecules, Coll. Surf.B. Biointerfac., 27,159-168 (2002).
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Hu FQ, Yuan H, Zhang HH, Fang M. Preparation of solid lipid nanoparticles with clobetasol propionate by a novel solvent diffusion method in aqueous system and physicochemical characterization, Int. J. Pharm. 239, 121–128 (2002).
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Jie L, Wen Hu., Huabing Chena Qian N, Huibi Xu, Xiangliang Y. Isotretinoin-loaded solid lipid nanoparticles with skin targeting for topical delivery, Int. J. Pharm., (2006).
Kenon AP, Schawartz M A. Drug stability, J. Am. Chem. Soc., 60, 2976-2981 (1983).
Kipp JK. The role of solid nanoparticle technology in the parenteral delivery of poorly water-soluble drugs, Int. J. Pharm. 284, 109–122 (2004).
Kwon HY, Lee JH, Choi SW, Jang Y, Kim JH. Preparation of PLGA nanoparticles containing estrogen by emulsification diffusion method, Colloid Surf. A 182, 123–130 (2001).
Lachman L, Lieberman HA, Kanig LJ. The Theory and Practice of Industrial Pharmacy, Varghese Publishing House Bombay, 1987.
Lamprecht A, Saumet JL. Lipid nanocarriers as drug delivery system for ibuprofen in pain treatment, Int. J. Pharm. 278, 407–414 (2004).
Li Y, Dong L, Chang AJX, Xue H. Preparation and characterization of solid lipid nanoparticles loaded traditional chinese medicine, Int. J. Biological Macromolecules, 38, 296–299 (2006).
Lippacher A, Muller RH, Mader K. Semisolid SLNs dispersions for topical application: influence of formulation and production parameters on viscoelastic properties, Eur.J.Pharm.Biopharm., 53, 155–160 (2002).
Lippachera A, Muller RH, Mader K. Liquid and semisolid SLNe dispersions for topical application rheological characterization, Eur. J.Pharm. and Biopharm., 58, 561–567 (2004).
Maia CS, Mehnert W, Korting MHF. Solid lipid nanoparticles as drug carriers for topical glucocorticoids, Int. J. Pharm., 196, 165–167 (2000).
Marengo Emilio, Cavalli Roberta, Caputo Otto, Rodriguez Lorenzo, Gasco Maria Rosa. Scale-up of the preparation process of solid lipid nanospheres Part I, Int. J. Pharm., 205, 3–13 (2000).
Mehnert W, Mader K. Solid lipid nanoparticles Production, characterization and applications, Adv. Drug Del. Rev., 47, 165–196 (2001).
Meia Z, Lia X, Wua Q, Hua S, Yang X. The research on the anti-inflammatory activity and hepatotoxicity of triptolide-loaded solid lipid nanoparticles, Pharmacol. Res., 51, 345–351 (2005).
Miglietta A, Cavalli R, Bocca C, Gabriel L, Gasco MR. Cellular uptake and cytotoxicity of solid lipid nanospheres (SLN) incorporating doxorubicin or paclitaxel, Int. J. Pharm., 210, 61–67 (2000).
Morel S, Ugazio E, Cavalli R, Gasco MR. Thymopentin in solid lipid nanoparticles, Int. J. Pharm., 132, 259 261 (1996).
Morela S, Terrenob E, Ugazioa E, Aimeb S, Gascoa MR. NMR relaxometric investigations of solid lipi nanoparticles (SLN) containing gadolinium (III) complexes, Eur. J. Pharm. Biopharm., 45, 157–163 (1998).
Muller RH, Maeder K, Gohla S. Solid lipid nanoparticles (SLN) for controlled drug delivery a review of the state of the art, Eur. J.Pharm. and Biopharm., 50,161-177 (2000).
Muller RH, Radtke M, Wissing SA. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations, Adv. Drug Deliv. Rev. 54 (1), S131–S155 (2002).
Muller RH, Rungea S, Ravelli V, Mehnert W, Thunemannc AF, Souto EB. Oral bioavailability of cyclosporine: Solid Lipid Nanoparticles (SLNs) versus drug nanocrystals, Int. J. Pharm. 317, 82–89 (2006).
Olbrich C, Gessner A, Kayser O, Muller RH. Lipid-drug conjugate (LDC) nanoparticles as novel carrier system for hydrophilic antitrypanosomal drug diminazenediaceturate, J. Drug Target. 10, 387–396 (2002).
Norouzi MR, Ganjali S, Labbafi A Mohammadi. Subsecond FFT-adsorptive Voltammetric Technique as a Novel Method for Subnano Level Monitoring of Piroxicam in its Tablets and Bulk Form at Au Microelectrode in Flowing Solutions, Analytical Letters, 40, 747-762 (2007).
Pandey R, Sharma S, Khuller GK. Tuberculosis: Oral solid lipid nanoparticle-based antitubercular chemotherapy, Tuberculosis. 85, 415–420 (2005).
Quintanar-Guerrero D, All´emann E, Doelker E, Fessi H. A mechanistic study of the formation of polymer nanoparticles by the emulsification-diffusion technique,Colloid. Polym. Sci. 275, 640–647 (1997).
Quintanar-Guerrero D, All´emann E, Fessi H., Doelker E. Preparation techniques and mechanisms of formation of biodegradable nanoparticles from preformed polymers, Drug Dev. Ind. Pharm. 24, 1113–1128 (1998a).
Quintanar-Guerrero D, Allemann E, Fessi H, Doelker E. Pseudolatex preparation using a novel emulsion-diffudion process involving direct displacement of partially water-miscible solvents by distillation, Int. J. Pharm. 188, 155–164 (1999b).
Quintanar-Guerrero D, Fessi H, Allemann E, Doelker E. Influence of stabilizing agents and preparative variables on the formation of poly (d,l-lactic acid) nanoparticles by an emulsification-diffusion technique, Int. J. Pharm. 143, 133–141 (1996).
Quintanar-Guerrero D, Ganem-Quintanar A, Allemann E, Fessi H, Doelker E. Influence of the stabilizer coating layer on the purification and freeze-drying of poly(d,l-lactic acid) nanoparticles prepared by an emulsion-diffusion technique, J. Microencapsul. 15, 107–119 (1998b).
Quintanar-Guerrero D, Gurny R, Allemann E, Fessi H, Doelker, E. Method for producing aqueous colloidal dispersions of nanoparticles. WO Patent No. 01,002,087 (1999a).
Reddy H, Murthy RSR. Etoposide-Loaded Nanoparticles Made from Glyceride Lipids: Formulation,Characterization, in Vitro Drug Release, and Stability Evaluation, AAPS, Pharm.Sci.Tech., 6(2), Article 24 (2005).
Saupe A, Gordon CK, Rades T. Structural investigations on nanoemulsions, solid lipid nanoparticles and nanostructured lipid carriers by cryo-field emission scanning electron microscopy and Raman spectroscopy, Int. J. Pharm., 314, 56–62 (2006).
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