RP-UFLC Method for Estimation of Propafenone in Tablets
DOI:
https://doi.org/10.37285/ijpsn.2014.7.4.9Abstract
A simple, precise and accurate RP-UFLC method was developed for determination of propafenone hydrochloride. Separation was carried on an Enable C18G column (250 mm × 4.6 mm i.d., 5 μm) using methanol: 10 mM TBAHS (95:05, v/v) as mobile phase at flow rate of 1.0 ml/min in isocratic mode. The PDA detection wavelength was 247 nm. The retention time of propafenone was 2.692 min. The method was validated for linearity, specificity, precision, accuracy and robustness as per ICH. The method was linear in the concentration range of 10 – 250 µg/ml with correlation coefficients of 0.999. The LOD and LOQ were 4.5 µg/ml and 9.75 µg/ml, respectively. Average recoveries for recovery study were found to be in the range of 99.73-100.58. R.S.D. values for intraday, interday and system precision were found to be less than 2%. Specificity was established after forced degradation was performed by using HCl, NaOH, H2O2, thermal and UV radiation. The method was applied successfully for estimation propafenone in tablet formulation.
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Keywords:
Propafenone, Reverse phase, Liquid chromatography, Forced degradation, Tablets
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